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MUSCLE is a program for creating multiple alignments of amino acid or nucleotide sequences. A range of options is provided that give you the choice of optimizing accuracy, speed, or some compromise between the two. Default parameters are those that gave the best average benchmark accuracy in my tests. However,.
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The primary output is specified using the -out option, and defaults to standard output. Multiple output formats in one run. You can specify several -xxxout option on the same command line, which allows you to create multiple output formats in a single run, e.g.: muscle -in seqs.fa -fastaout seqs.afa -clwout seqs.aln
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Popular multiple alignment software. MUSCLE is one of the most widely-used methods in biology. On average, MUSCLE is cited by ten new papers every day. Fast, accurate and easy to use. MUSCLE is one of the best-performing multiple alignment programs according to published benchmark tests, with accuracy and
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